| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human TFIIE alpha was used as the immunogen for the GTF2E1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GTF2E1 Antibody / TFIIE alpha is a anti-GTF2E1 Rabbit antibody Recombinant Rabbit Monoclonal clone 31G53 supplied in Liquid format. Recommended for workflows such as Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GTF2E1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31G53, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): IF, IHC, ICC, WB
Biological background
General transcription factor IIE subunit 1 is essential for formation of the preinitiation complex. Research using GTF2E1 antibody has shown that TFIIE alpha interacts directly with TFIIH, stabilizing its association with RNA polymerase II. This promotes ATP dependent unwinding of DNA and formation of the open complex, a prerequisite for RNA synthesis. Without TFIIE alpha, transcription initiation is severely impaired, highlighting its fundamental role in gene expression.
Beyond its structural role, GTF2E1 has regulatory functions. Studies with GTF2E1 antibody have revealed that TFIIE alpha influences the kinase activity of TFIIH, modulating phosphorylation of the C terminal domain of RNA polymerase II. This links TFIIE to transcriptional regulation and elongation. Because transcription is tightly coupled to cell cycle progression and differentiation, TFIIE alpha plays broad roles in physiology and development.
Mutations and altered expression of GTF2E1 have been associated with developmental abnormalities and cancer. Research using GTF2E1 antibody has shown that defects disrupt transcriptional fidelity, leading to gene expression changes that contribute to disease. Its role in basal transcription makes it a potential biomarker for transcriptional dysregulation in oncology and genetic disorders.
GTF2E1 antibody is widely applied in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting demonstrates expression in proliferative tissues, immunohistochemistry reveals nuclear localization, and immunoprecipitation identifies interaction with TFIIH. These applications make GTF2E1 antibody essential in transcription biology.
By supplying validated GTF2E1 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
