| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Granzyme M/GZMM was used as the immunogen for the GZMM antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
GZMM Antibody / Granzyme M is a anti-GZMM Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC) with listed reactivity in Human.
Key elements and design rationale
- Target: GZMM
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): IHC
Biological background
Functionally, GZMM antibody identifies a 262-amino-acid protein localized to cytotoxic granules of NK and CD8+ T cells. Granzyme M is stored in secretory vesicles and released upon immune activation to cleave intracellular substrates in infected or malignant cells. It works synergistically with perforin and other granzymes to initiate rapid target cell lysis.
The GZMM gene is located on chromosome 19p13.3 within the granzyme gene cluster and is expressed predominantly in NK cells and cytotoxic T lymphocytes. GZMM contributes to immune surveillance by degrading viral and bacterial proteins and modulating proinflammatory signaling pathways.
Pathologically, elevated GZMM activity has been observed in inflammatory and autoimmune diseases, as well as in cancer immune responses. Deficiency impairs cytotoxic clearance of pathogens and tumor cells. Research using GZMM antibody supports studies in immunology, cytotoxic signaling, and inflammation control.
GZMM antibody is validated for western blotting, flow cytometry, and immunohistochemistry to detect cytotoxic granule enzymes.
Structurally, Granzyme M is a trypsin-like serine protease containing the catalytic triad His-Asp-Ser and a substrate-binding pocket that determines cleavage specificity. This antibody aids in examining GZMM's role in cytotoxic defense and immune regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
