| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human H1-7 recombinant protein (Position: H35-R130) was used as the immunogen for the H1-7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
H1-7 Antibody / Histone H1.7 is a anti-H1-7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: H1-7
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
H1.7 is expressed almost exclusively in testicular germ cells, particularly in pachytene spermatocytes and round spermatids, where chromatin undergoes extensive reorganization. It replaces somatic H1 variants during meiotic and post-meiotic stages, allowing transcriptional flexibility essential for spermatogenic gene expression. H1.7 contributes to chromatin condensation preceding the histone-to-protamine transition that culminates in sperm chromatin packaging. The developmental restriction and unique structural properties of H1.7 make it an important model for studying chromatin dynamics and gene regulation in reproductive cells.
The H1-7 antibody is extensively used in chromatin biology and reproductive research to detect this testis-specific linker histone. Western blot analysis typically identifies a 22 kilodalton band corresponding to H1.7, while immunohistochemistry reveals nuclear localization in spermatocytes and spermatids. Expression of H1.7 is temporally regulated during spermatogenesis, peaking in mid- to late-meiotic cells. The antibody aids in distinguishing stages of germ cell development and in analyzing chromatin remodeling events that occur during sperm maturation.
Beyond reproduction, studies of H1.7 provide insights into histone variant specialization and epigenetic regulation. Its distinct sequence and phosphorylation sites confer specialized interactions with chromatin remodeling complexes and transcriptional regulators. Comparative analyses using the H1-7 antibody reveal how testis-specific histones maintain genomic integrity while supporting dynamic transcription in germ cells.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
