| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human HADHA recombinant protein (Position: R20-N758) was used as the immunogen for the HADHA antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HADHA Antibody / Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha is a anti-HADHA Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).
Key elements and design rationale
- Target: HADHA
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, IHC, ICC, WB
Biological background
Functionally, HADHA antibody recognizes a 79 kDa protein localized to the inner mitochondrial membrane, where it catalyzes key reactions in the beta-oxidation pathway of long-chain fatty acids. HADHA converts long-chain enoyl-CoA substrates to hydroxyacyl-CoA and subsequently to 3-ketoacyl-CoA intermediates, producing NADH and acetyl-CoA for energy generation. This process is vital for sustaining ATP production in tissues with high oxidative energy requirements, including heart, skeletal muscle, and liver. Disruption of HADHA function impairs mitochondrial fatty acid oxidation, leading to metabolic imbalance and accumulation of toxic lipid intermediates.
The HADHA gene is located on chromosome 2p23.3 and encodes one of the two subunits forming the hetero-octameric mitochondrial trifunctional protein complex. HADHA and HADHB assemble into four alpha-beta dimers that operate cooperatively in fatty acid oxidation. The enzyme's activity is critical during fasting and prolonged exercise when fatty acids serve as the major energy source. Mutations in HADHA result in long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency (LCHAD deficiency), a metabolic disorder characterized by hypoglycemia, hepatic dysfunction, and cardiomyopathy.
HADHA antibody is used to investigate mitochondrial metabolism, lipid oxidation, and energy regulation. It is particularly valuable in studies of metabolic disorders, mitochondrial dysfunction, and lipid-associated diseases. In cellular research, HADHA serves as a mitochondrial marker and is often co-stained with oxidative phosphorylation proteins to assess organelle function. Reduced HADHA expression is associated with defective energy metabolism in diabetes, fatty liver disease, and heart failure. Conversely, upregulation may indicate compensatory enhancement of fatty acid catabolism under stress.
Structurally, HADHA contains an N-terminal enoyl-CoA hydratase domain and a C-terminal 3-hydroxyacyl-CoA dehydrogenase domain, both dependent on NAD+ for catalytic turnover. Post-translational regulation of HADHA involves acetylation, phosphorylation, and interactions with acyl-CoA binding proteins that modulate substrate specificity. Its proper function is essential for maintaining mitochondrial redox balance and preventing lipid-induced oxidative stress.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
