| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human HAND1 was used as the immunogen for the HAND1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HAND1 Antibody / Heart and neural crest derivatives-expressed protein 1 is a anti-HAND1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: HAND1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB
Biological background
Functionally, HAND1 antibody identifies a 215-amino-acid nuclear protein that binds E-box DNA motifs (CANNTG) as a heterodimer with other bHLH transcription factors such as E12 and HAND2. HAND1 activates or represses transcription depending on partner interactions and chromatin context. During embryogenesis, HAND1 is expressed in the developing heart, extraembryonic tissues, and craniofacial mesenchyme, where it directs differentiation of myocardial, trophoblast, and smooth muscle lineages.
The HAND1 gene is located on chromosome 5q33.2 and is transcriptionally regulated by cardiac transcription factors including NKX2-5, GATA4, and MEF2C. HAND1 functions in a dosage-sensitive manner, as either loss or overexpression disrupts normal cardiac looping and ventricular formation. It acts downstream of BMP and WNT signaling pathways that orchestrate early mesoderm and cardiac progenitor specification.
In the heart, HAND1 contributes to left ventricular development and patterning of the outflow tract. It regulates expression of structural and contractile genes required for cardiomyocyte differentiation. In the placenta, HAND1 promotes trophoblast giant cell differentiation and invasion, critical for maternal-fetal exchange. Beyond development, HAND1 has been implicated in cancer biology, where aberrant expression can influence cell proliferation, migration, and angiogenesis through transcriptional reprogramming of developmental pathways.
HAND1 antibody is widely used in developmental biology, cardiology, and transcriptional regulation research. It is suitable for immunohistochemistry, immunofluorescence, and western blotting to detect HAND1 expression in embryonic and differentiated tissues. This antibody supports studies of cardiac gene regulation, lineage specification, and embryonic morphogenesis. It also aids in placental biology research and models of congenital heart defects.
Structurally, HAND1 contains a basic DNA-binding region and a helix-loop-helix dimerization motif that enable sequence-specific DNA binding and partner selection. Post-translational modifications such as phosphorylation influence HAND1 dimerization and transcriptional output.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
