| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human HAPLN1 recombinant protein (Position: D16-N354) was used as the immunogen for the HAPLN1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HAPLN1 Antibody / Hyaluronan and proteoglycan link protein 1 is a anti-HAPLN1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: HAPLN1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
HAPLN1 is encoded by the HAPLN1 gene on human chromosome 5q14.3. The protein is approximately 38 kilodaltons and belongs to the link protein family characterized by tandem immunoglobulin-like domains. HAPLN1 forms ternary complexes with hyaluronan and aggrecan, contributing to the viscoelastic properties of cartilage and the structural framework of the brain extracellular matrix. It is critical for the assembly of perineuronal nets, specialized ECM structures that regulate neuronal excitability and plasticity.
Using the HAPLN1 antibody, researchers can detect the protein by western blot, where it appears as a 38�40 kilodalton band, or by immunohistochemistry, where it localizes to extracellular matrix regions of cartilage, brain, and developing tissues. HAPLN1 expression begins early in embryogenesis, marking sites of cartilage condensation and neural patterning. In adults, it contributes to the stability of mature ECM networks, particularly within articular cartilage and neuronal environments. Loss or alteration of HAPLN1 disrupts ECM cohesion, leading to joint instability or impaired neuronal function.
HAPLN1 has clinical relevance in osteoarthritis, rheumatoid arthritis, and neurodegenerative diseases. Decreased HAPLN1 levels correlate with cartilage degradation and ECM disorganization. In the nervous system, reduced expression is linked to increased synaptic plasticity and neuroinflammation. Conversely, HAPLN1 overexpression may promote fibrotic ECM accumulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
