| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GSG2/HASPIN recombinant protein (Position: R86-K798) was used as the immunogen for the HASPIN antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HASPIN Antibody / Serine/threonine-protein kinase haspin is a anti-HASPIN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: HASPIN
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Serine/threonine-protein kinase haspin is an atypical protein kinase with unique structural features distinguishing it from conventional kinase families. It remains active during mitosis and localizes to chromatin, where it marks centromeric regions to coordinate kinetochore function. The HASPIN antibody allows visualization of its distribution within dividing cells and quantification of total or active forms. This enables assessment of how Haspin integrates chromatin modification with spindle checkpoint signaling to maintain genomic stability.
Phosphorylation of histone H3 by Haspin serves as a molecular signal that recruits the Aurora B-containing CPC complex, which is essential for correcting improper microtubule-kinetochore attachments. Loss of Haspin activity disrupts chromosome segregation and leads to aneuploidy. The HASPIN antibody has therefore become a standard reagent for cell cycle and chromosome biology research, particularly in studies of mitotic kinase cascades and epigenetic checkpoint control.
In addition to its role in mitosis, Serine/threonine-protein kinase haspin has been implicated in spermatogenesis and embryonic development. Its expression in germ cells suggests functions in chromatin condensation and meiotic progression. The HASPIN antibody supports examination of these developmental contexts, providing insights into how histone phosphorylation contributes to chromatin organization beyond mitosis.
Given the importance of Haspin in maintaining genomic stability, it has emerged as a potential target for anticancer therapy. Inhibitors that block its kinase activity sensitize tumor cells to mitotic stress and disrupt proliferation. Researchers use the HASPIN antibody to evaluate target engagement and monitor cellular responses to Haspin inhibition.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
