| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human UCHL5IP was used as the immunogen for the HAUS7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HAUS7 Antibody / UCHL5IP is a anti-HAUS7 Rabbit antibody Recombinant Rabbit Monoclonal clone 30H47 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) with listed reactivity in Human.
Key elements and design rationale
- Target: HAUS7
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30H47, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, IP
Biological background
HAUS7 antibody is widely used in cell biology and cancer research. The augmin complex promotes branching microtubule nucleation, a mechanism essential for robust spindle assembly in dividing cells. By detecting HAUS7, researchers can study how spindle architecture is generated and maintained. Disruption of augmin components leads to spindle defects, chromosome missegregation, and aneuploidy, processes often linked to tumorigenesis.
Western blot assays detect HAUS7 protein bands in dividing cell extracts. Immunohistochemistry highlights HAUS7 expression in proliferative tissues, while immunofluorescence maps localization at mitotic spindles. These approaches allow detailed analysis of augmin function in cell division.
HAUS7 and other augmin subunits play roles in neural stem cell division, embryonic development, and tissue regeneration. Their contribution to faithful chromosome segregation highlights their importance for preventing genomic instability and disease. By applying HAUS7 antibody, scientists can investigate mechanisms linking spindle regulation to cancer and developmental disorders.
HAUS7 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
