| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human HBP1 recombinant protein (Position: D51-R506) was used as the immunogen for the HBP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HBP1 Antibody / HMG-box transcription factor 1 is a anti-HBP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: HBP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Structurally, HBP1 is a 513-amino-acid nuclear protein of approximately 60 kilodaltons containing an HMG-box DNA-binding domain and transcriptional repression domains that interact with histone deacetylases (HDACs) and chromatin remodeling complexes. These interactions enable HBP1 to repress genes such as CCND1 (Cyclin D1) and N-MYC, thereby inhibiting uncontrolled cell proliferation. HBP1 also contains regulatory phosphorylation sites that control nuclear localization and DNA-binding affinity in response to extracellular cues.
The HBP1 antibody is widely used in cell cycle, cancer, and transcription research to study gene repression, differentiation, and tumor suppression mechanisms. Western blot analysis detects a 60 kilodalton band corresponding to HBP1, while immunofluorescence shows strong nuclear localization in epithelial and neuronal cells. This antibody enables detailed examination of transcriptional networks controlling growth arrest and lineage commitment.
Functionally, HBP1 suppresses oncogenic transformation by inhibiting Wnt/beta-catenin signaling and antagonizing MYC-dependent transcription. It also promotes senescence and apoptosis in response to DNA damage and oxidative stress, acting as a barrier to tumorigenesis. Reduced HBP1 expression or mutation is associated with breast, lung, and colon cancers, where loss of repression contributes to hyperproliferation. Beyond cancer, HBP1 regulates differentiation programs in neurons, adipocytes, and muscle cells by coordinating chromatin remodeling and transcriptional silencing. The HBP1 antibody provides a valuable tool for exploring these pathways and assessing transcriptional regulation in normal and diseased tissues.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
