| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human CECR5 was used as the immunogen for the HDHD5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HDHD5 Antibody / Haloacid dehalogenase-like hydrolase domain-containing 5 / CECR5 is a anti-HDHD5 Rabbit antibody Recombinant Rabbit Monoclonal clone 29H91 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.
Key elements and design rationale
- Target: HDHD5
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29H91, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF
Biological background
The HAD superfamily includes enzymes that act on a variety of phosphorylated substrates, ranging from sugars to nucleotides. Research using HDHD5 antibody has revealed that Haloacid dehalogenase-like hydrolase domain-containing protein 5 contains the signature catalytic motifs typical of HAD enzymes, suggesting conserved catalytic potential. Structural studies indicate that it adopts the canonical HAD fold, characterized by a Rossmann-like core and conserved aspartate residues required for catalytic activity. These features strongly suggest that HDHD5 has enzymatic activity related to phosphatase or hydrolase function, though the precise substrate remains under investigation.
Genetic studies have linked HDHD5 to the Cat Eye syndrome critical region on chromosome 22, where the CECR5 gene is located. Research using HDHD5 antibody has suggested that altered expression of this protein may contribute to phenotypic features of this syndrome, which is characterized by congenital anomalies involving the eyes, heart, and skeletal system. Although the exact contribution of Haloacid dehalogenase-like hydrolase domain-containing protein 5 to Cat Eye syndrome remains unclear, its chromosomal location and potential enzymatic activity make it a protein of strong biomedical interest.
In addition to developmental disorders, emerging evidence suggests that HDHD5 may play roles in RNA regulation and cellular stress responses. Studies with HDHD5 antibody have demonstrated that the protein is expressed in multiple tissues, with particularly high expression in metabolically active organs. This distribution pattern supports a potential role in nucleotide metabolism or signaling. Because many HAD superfamily members regulate metabolic flux, Haloacid dehalogenase-like hydrolase domain-containing protein 5 may influence pathways tied to energy balance and gene expression control.
HDHD5 antibody is a versatile reagent applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms protein size and expression across tissues, immunohistochemistry highlights tissue-specific localization, and immunofluorescence reveals its subcellular distribution, which appears to be both cytoplasmic and nuclear. These applications make HDHD5 antibody valuable for investigating its role in metabolism, signaling, and development.
Functional studies with HDHD5 antibody are beginning to clarify its interaction network. Evidence suggests that Haloacid dehalogenase-like hydrolase domain-containing protein 5 may interact with RNA binding proteins or nucleotide processing enzymes, supporting a potential function in RNA maturation or degradation. Its presence in both cytoplasmic and nuclear compartments further suggests a dual role in metabolic and genetic regulation. These observations position HDHD5 as an emerging regulator in pathways not yet fully defined but of significant biomedical importance.
By supplying validated HDHD5 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
