| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Host | |
| Product Format | 1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium |
| Product Type | |
| Selection Marker | Puromycin (1 μg/mL) |
| Species | |
| Storage | |
| Target |
Scientific Background
Stable cell lines that constitutively express a defined target antigen provide a reproducible, renewable tool for antibody characterization, binding studies, and cell-based screening. This line expresses CD274/PD-L1/B7-H1 (Human) on a well-characterized HEK293T background, enabling consistent flow-cytometric detection against an untransfected control.
Stable Cell LineProduct Description
HEK293T Human CD274/PD-L1/B7-H1 Stable Cell Line is a HEK293T-derived stable cell line engineered to constitutively express Human CD274/PD-L1/B7-H1 under Puromycin selection. The line grows as an adherent culture and is validated by flow cytometry, making it suitable for antibody binding/blocking assays, FACS-based screening, and target-expression studies.
Specifications
| Host species | HEK293T |
|---|---|
| Target | CD274/PD-L1/B7-H1 |
| Species | Human |
| Accession | Q9NZQ7 |
| Growth Properties | Adherent |
| Selection Marker | Puromycin (1 μg/mL) |
| Applications | Flow Cytometry |
| Specifications | 1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium |
| Stability and Storage | Frozen in liquid nitrogen. |
Quality Control & Validation
Validated by Flow Cytometry
Target expression is confirmed by flow-cytometric staining of the transfected line versus untransfected HEK293T control cells (see product image).
Usage Notes
Supplied as 1 vial contains at least 2×10^6 cells in 1 ml freeze medium. Thaw rapidly at 37 °C, transfer to pre-warmed complete growth medium, and maintain under standard mammalian culture conditions (37 °C, 5% CO₂). Maintain Puromycin selection to preserve stable expression. Expand and re-cryopreserve early-passage stocks.
Safety & Handling
Handle as potentially biohazardous mammalian cell material in a certified biosafety cabinet following your institutional guidelines. Wear appropriate PPE and decontaminate all waste before disposal.
Regulatory
For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures.
Related Products
Explore additional stable cell lines, recombinant proteins, and antibodies against the same target family available through BioHippo.
- Cell line sourcing and selection (species, tissue, and disease model matching)
- Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA)
- Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs)
- Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles)
- Inducible expression systems (Tet-On/Off and regulatable constructs)
- Drug resistance marker selection (puromycin, G418, hygromycin, and others)
- Custom growth and media optimisation for specific assay requirements
- Scale-up production for high-throughput screening campaigns
- Authentication and QC services (STR profiling, mycoplasma testing, viability assessment)
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