| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human HERC4 recombinant protein (Position: D18-I1057) was used as the immunogen for the HERC4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HERC4 Antibody / HECT domain and RCC1-like domain-containing protein 4 is a anti-HERC4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: HERC4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
HERC4 expression is widespread but most pronounced in testis, brain, and skeletal muscle. Functional studies have demonstrated roles in germ cell differentiation and embryonic development. In the testis, HERC4 localizes to elongating spermatids and regulates ubiquitin-dependent remodeling of proteins essential for sperm maturation. Loss of HERC4 in model organisms leads to male infertility and abnormal spermatid morphology, confirming its requirement for spermatogenesis. In somatic tissues, HERC4 participates in protein quality control and signaling regulation, including modulation of TGF-beta and Wnt pathways.
The HERC4 antibody is commonly used in cell biology and reproductive biology research to detect HERC4 protein expression and localization. Western blot analysis typically reveals a 120 kilodalton band corresponding to the ligase, while immunofluorescence shows cytoplasmic and perinuclear localization consistent with its role in ubiquitin-mediated trafficking. Elevated HERC4 expression has been reported in several cancer types, including breast and liver carcinoma, where it promotes degradation of tumor suppressors and supports proliferation. Because HERC4 also influences mitochondrial dynamics, the antibody is valuable for studies examining proteostasis, signaling, and metabolism.
Mechanistically, HERC4 acts through its HECT domain to catalyze the formation of polyubiquitin chains, often targeting regulatory proteins involved in stress response and apoptosis. It interacts with molecular chaperones and ubiquitin ligase adaptors to fine-tune degradation specificity. Dysregulation of HERC4 activity leads to aberrant protein accumulation and altered signaling balance. The HERC4 antibody is therefore a versatile tool for exploring E3 ligase function in development, reproduction, and cancer biology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
