| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse HEXIM2 recombinant protein (Position: L17-R313) was used as the immunogen for the HEXIM2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HEXIM2 Antibody / Hexamethylene bisacetamide-inducible protein 2 is a anti-HEXIM2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: HEXIM2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, HEXIM2 antibody identifies a 286-amino-acid protein localized predominantly in the nucleus, where it binds 7SK small nuclear RNA (7SK snRNA) to form the 7SK snRNP complex. This complex sequesters and inhibits P-TEFb, thereby controlling the phosphorylation of the C-terminal domain (CTD) of RNA polymerase II and modulating gene expression rates. HEXIM2 dynamically regulates the transition between transcriptional pausing and elongation, coordinating cellular responses to stress, differentiation, and growth factors.
The HEXIM2 gene is located on chromosome 17q21.32 and is expressed in various tissues, including brain, heart, and skeletal muscle. It plays important roles in transcriptional control during development and cell cycle progression, as well as in regulating expression of immediate early genes and viral transcriptional machinery.
Pathologically, altered HEXIM2 expression has been implicated in cancer, cardiac hypertrophy, and neurodegenerative diseases. Decreased HEXIM2 function enhances P-TEFb activity, leading to hyperactive transcription and dysregulated cell growth, while increased expression contributes to transcriptional repression and altered differentiation. Research using HEXIM2 antibody supports studies in transcriptional regulation, RNA biology, and disease-related gene expression control.
HEXIM2 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect nuclear transcriptional regulators.
Structurally, Hexamethylene bisacetamide-inducible protein 2 contains an RNA-binding domain and basic regions essential for 7SK snRNA interaction and P-TEFb inhibition. Its conformational flexibility allows rapid response to signaling cues that release or restore inhibition of transcription elongation. This antibody enables detailed investigation of HEXIM2's function in controlling RNA polymerase II-mediated transcription and cellular adaptation to stress.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
