| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A human recombinant partial protein (amino acids M1-K789) was used as the immunogen for the Hexokinase 3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Hexokinase 3 Antibody / HK3 is a anti-HK3 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P, Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasmic, extracellular.
Key elements and design rationale
- Target: HK3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P, FACS, ELISA
Biological background
HK3 differs from other hexokinase isoforms in its tissue distribution and regulatory properties. It is predominantly expressed in leukocytes and other hematopoietic cells, suggesting a specialized role in immune cell metabolism. Unlike HK1 and HK2, HK3 does not strongly associate with the outer mitochondrial membrane, which may influence its role in apoptosis and energy regulation. Employing a Hexokinase 3 antibody allows scientists to explore its unique expression patterns and its contributions to both glycolysis and immune cell biology.
Altered expression of HK3 has been observed in cancer and inflammatory conditions, where shifts in glucose metabolism play a role in disease progression. HK3 has also been implicated in oxidative stress responses and metabolic reprogramming of immune cells. As interest in immunometabolism continues to grow, HK3 remains an important protein for research into metabolic regulation and disease. Using a Hexokinase 3 antibody enables reliable detection of this isoform in various biological contexts.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
