| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human HIBADH recombinant protein (Position: R34-E332) was used as the immunogen for the HIBADH antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HIBADH Antibody / 3-Hydroxyisobutyrate dehydrogenase is a anti-HIBADH Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: HIBADH
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
HIBADH localizes to the mitochondrial matrix, where it functions within the valine degradation pathway that ultimately generates succinyl-CoA for entry into the TCA cycle. This enzymatic step contributes to both energy generation and metabolic flexibility, especially during fasting or muscle exertion. In addition to its metabolic role, HIBADH helps regulate mitochondrial redox balance through NAD+/NADH interconversion. Mutations or deficiencies in this enzyme may disrupt amino acid catabolism and contribute to metabolic disorders involving organic acid accumulation.
The HIBADH antibody is used in metabolism, mitochondrial, and enzymology research to detect mitochondrial dehydrogenases and characterize amino acid catabolic pathways. Western blot analysis identifies a 35 kilodalton band corresponding to HIBADH, while immunofluorescence reveals punctate mitochondrial staining. This antibody provides a valuable reagent for studying mitochondrial metabolism, oxidative phosphorylation, and amino acid utilization under physiological and disease conditions.
Beyond energy metabolism, altered HIBADH expression has been linked to mitochondrial dysfunction, muscle wasting, and cancer metabolism. Its regulation reflects cellular metabolic adaptation to nutrient and energy demands. The HIBADH antibody is validated by
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
