| Field | Specification |
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Product Overview
This HIF-1-Alpha isoform 1 + 2 Positive Control Protein provides a reliable known-positive sample for validating anti-HIF-1-Alpha isoform 1 + 2 antibody performance in Western blot and IHC applications. Use alongside experimental samples to confirm detection conditions before interpreting results in novel sample types or with a new antibody lot. Paired antibody: STJ501335.
Target & Specificity
| UniProt ID | Q16665 |
|---|
This is positive control is recommended for use in combination with HIF-1-Alpha isoform 1 + 2 antibody STJ501335.
Specifications
| Formulation | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli s buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% b |
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| Storage | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Validated Applications | Western Blot |
| Recommended Dilution | WB: 1:500 |
Recommended Use
For WB: load 5–20 ng per lane alongside experimental samples to confirm antibody function and expected band size. For IHC: apply to a positive-control tissue section at the concentration recommended for the paired antibody.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorSt John's Laboratory LtdCatalog No.STJ504236UniProtQ16665
Load 5–20 ng per WB lane alongside experimental samples to confirm anti-HIF-1-Alpha isoform antibody performance. For IHC, apply to a positive-control tissue section alongside experimental sections. Pair with antibody STJ504236 for matched validation.
For WB: 5–20 ng per lane produces a clear band under standard conditions. For IHC: use the concentration specified on the antibody datasheet. If no signal: confirm antibody is within expiry and antigen retrieval conditions are optimal.
This peptide is designed to pair with antibody STJ504236. Using with a different antibody clone may not produce blocking results if epitopes differ.
Validated for Western Blot specificity controls. Performance in other applications (ELISA, IP) requires independent validation.
Store at-20°C for long term storage. Avoid freeze-thaw cycles.. Prepare single-use aliquots to avoid freeze-thaw; stable ≥12 months at −20 °C when lyophilized.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
