| Field | Specification |
|---|---|
| Alternative Names | Hypoxia-inducible factor 1-alpha (HIF-1-alpha, HIF1-alpha)|ARNT-interacting protein|Basic-helix-loop-helix-PAS protein MOP1|Class E basic helix-loop-helix protein 78 (bHLHe78)|Member of PAS protein 1|PAS domain-containing protein 8|HIF1A|BHLHE78|MOP1|PASD8 |
| Clonality | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Product Overview
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
Polyclonal Rabbit AntibodyValidated Applications
| Application | Recommended Dilution |
|---|---|
| ELISA | Refer to datasheet |
| Immunohistochemistry | Refer to datasheet |
| Western Blot | Refer to datasheet |
| Immunofluorescence | Refer to datasheet |
Immunogen & Specificity
Immunogen: hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)
UniProt/Swiss-Prot: Q16665
Product Specifications
| Host Species | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Species Reactivity | Human, Mouse |
| Purification Method | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Molecular Weight | 120 kDa |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
Reactivity Notes
Confirmed reactivity in Human, Mouse samples; validated for ELISA, Immunohistochemistry, Western Blot, Immunofluorescence.
Safety & Handling
- For Research Use Only (RUO). Not intended for diagnostic procedures.
- Shipping: cold-chain shipment (typically with ice packs).
- Upon receipt: store at recommended temperature as soon as possible.
- Sales terms and conditions: Please review prior to ordering.
Related Products
Explore additional antibodies and related detection reagents for HIF1A in the BioHippo catalog.
HIF1A antibody
First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.
Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.
Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.
Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.
Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).
Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)
Heat induced antibody retrieval:
Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;
Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.
BioHippo can support custom antibody formatting and service options for selected products. Contact us to discuss your project requirements.
Potential customization options include:
- Custom conjugation: fluorophore, biotin, enzyme, or nanoparticle labeling available on request for qualified antibodies.
- Bulk or scale-up quantities: volume pricing and custom lead time estimates available for larger orders.
- Alternative formulations: azide-free, carrier protein-free, or lyophilized formats available upon request for selected products.
- OEM and private-label supply: custom packaging and branding available for commercial applications.
Submit your project specifications via the product contact form or reach us at support@ebiohippo.com for a tailored quotation.
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets
20% Off Transmembrane Proteins
50% Off Lab Consumables + Free Shipping
$99 Pipette Filler Promotion Package
BlasTaq 2X qPCR MasterMix - 50% OFF Limited Time Offer
DENARASE® Endonuclease — 10% Off One Order
10% OFF CELL LINES-Limited-Time Offer
