| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
Hypoxia-inducible factor 2 alpha (HIF-2α), encoded by EPAS1, is an oxygen-sensitive transcription factor that mediates cellular adaptation to low oxygen. Under normoxia, HIF-2α is hydroxylated and targeted for degradation; under hypoxia it is stabilized, dimerizes with HIF-1β, and binds hypoxia-response elements (HRE) to activate target genes. HIF-2α regulates erythropoiesis, angiogenesis, iron metabolism, and stem cell maintenance, with a tissue distribution and gene set partly distinct from HIF-1α. Dysregulated HIF-2α activity contributes to clear cell renal carcinoma and other tumors, making it an important target in oncology, angiogenesis, and hypoxia research.
Product Description & Applications
The HIF2a Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem hypoxia-response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of HIF-2α (EPAS1) activity in transduced cells. Reporter options include GFP, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Purified by PEG precipitation and sucrose gradient centrifugation, the particles efficiently transduce difficult-to-transfect cells, including primary and thawed cells.
Applications include monitoring hypoxia pathway activation, screening HIF-2α modulators, and studying angiogenesis and tumor biology.
About This Product
This reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Hypoxia/HIF pathway (HIF-2a) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
