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Description
Hifair™ Lyo-Ready I Multiplex One Step RT-qPCR Kit（UDG plus) is an optimized reagent for fast and sensitive amplification and quantitation of RNA targets with probe-based assays. In the process of the experiment, reverse transcription and quantitative PCR were carried out in the same tube, which simplified the experimental operation and reduced the risk of contamination.The unique Buffer and enzyme design ensures that it can be used in the preparation and product design of one-step RT-qPCR lyophilized reaction systems.
The kit mainly contains Hifair™ V Reverse Transcriptase, Hieff UniconTM HotStart Taq DNA Polymerase and optimized lyophilizable buffer,etc. In addition, the factors that can effectively inhibit the non-specific PCR amplification and improve the amplification efficiency of multiple qPCR reactions are added, which can ensure the amplification efficiency and carry out up to multiple amplification reaction.
This kit is fitted with lyoprotectant that can be used for lyophilization.
Features
- Broad Compatibility: Compatible with both direct-use and freeze-dried formats for long-term storage
- High Stability: Maintains excellent stability after lyophilization, even when stored at 50°C for up to 33 days
- Flexible Lyophilization Formats: Supports various formats including 8-tube strip in-situ lyophilization, vial-based lyophilization, and micro-core freeze-dried forms
- Rapid Workflow: Delivers results in as fast as 40 minutes with an optimized fast-cycling protocol
- Effective Contamination Control: Incorporates a UDG/dUTP system for efficient prevention and removal of carryover contamination
Application
Virus detection; pathogen detection; gene expression analysis;
Specifications
|
Cat.No. |
16645ES60 / 16645ES80 / 16645ES92 |
|
Size |
100 T / 1000 T / 10000 T |
Components
|
Components No. |
Name |
16645ES60 (100 T) |
16645ES80 (1000 T) |
16645ES92 (10,000 T) |
|
16645-A |
5×Hifair™ Lyo-Buffer |
500 μL |
5 mL |
50 mL |
|
16645-B |
Hifair™ Lyo-Enzyme Mix |
100 μL |
1 mL |
10 mL |
|
16645-C |
Lyoprotectant |
800 μL |
8 mL |
80 mL |
【Note】: 1) 5×Hifair™ Lyo-Buffer is the abbreviation of Lyo-Ready I Multiplex One Step RT-qPCR kit.
2) Hifair™ Lyo-Enzyme Mix mainly contains heat-resistant Hifair™ V Reverse Transcriptase, UNICONTM HotStart Taq DNA Polymerase and UDGase.
Shipping and Storage
This product should be stored at -25~-15℃ for one years. It should avoid repeated freezing and thawing.
Figures
1. Freeze-dried products in various forms
Figure 1. Freeze-dried product form
In situ freeze-drying and freeze-dried microspheres. The freeze-dried morphology was good after freeze-drying, there was no collapse on the in situ freeze-dried surface, and there was no gap in the tube; the freeze-dried microspheres were plump and smooth in shape and uniform in size.
2. Consistent performance
Figure 2. Consistent performance between Liquid reagent and lyophilized Microspheres
3. Stability test
Figure 3. Freeze-dried microspheres accelerate stability test
After being freeze-dried into freeze-dried microspheres using Yeasen's RNA lyophilizable reagent (Catt#16645ES), placed under different conditions (accelerated at 50°C for 33 days), and 104, and 103 copies/mL of HPIV pseudovirus templates were amplified simultaneously. The results showed that there was no significant change in the Ct value, fluorescence value, spherical shape, etc. of the freeze-dried microspheres before and after thermal acceleration, and the freeze-dried product had good stability.
4. Mutiplex PCR Amplification of pathogens
Figure 4. Multiplex amplification of pathogens
(HPIVs, Template concentration E6/5/4/3/2/1)
Documents:
Safety Data Sheet
Manuals
16645_Manual_Ver.EN20250520.pdf
Citations & References:
Related blog:
How to choose drying reagents, Air Drying or Lyophilization?
Validated RNA input typically ranges from 1 pg to 100 ng total RNA per reaction, depending on target abundance. For low-abundance viral RNA, use the lowest input guidance in the datasheet. Pre-treat samples with DNase I if genomic DNA contamination is suspected, particularly for targets without intron-spanning primers.
Yes. Kits designated "(UDG Plus)" incorporate Uracil-DNA Glycosylase (UDG) to enzymatically degrade dUTP-containing PCR carryover contaminants before amplification. This is especially critical in multiplex diagnostic settings where high sample throughput increases carryover risk. UDG is inactivated by heating during the reverse transcription step.
Yes. This kit is specifically designed for multiplex one-step RT-qPCR using probe-based detection (TaqMan-compatible), allowing simultaneous detection of multiple viral RNA targets in a single tube. Internal controls can be co-amplified without reconfiguring the assay. Consult the datasheet for validated multiplex panel configurations and instrument compatibility.
This kit is compatible with major probe-based qPCR platforms including Applied Biosystems QuantStudio, Bio-Rad CFX, Roche LightCycler, and equivalent instruments with multiplex fluorescence channel capability. Ensure your instrument supports the fluorophores used in your probe panel (typically FAM, HEX/VIC, ROX, Cy5). Instrument-specific thermocycling protocols are provided in the datasheet.
Select variants of this kit (e.g., "Lyo-Ready" designations) are specifically formulated for lyophilization compatibility or room-temperature shipping to facilitate point-of-care or field deployment. Standard liquid-format kits require –20°C storage. Consult the specific product datasheet for the validated reconstitution protocol if a lyophilized format is ordered.
Yeasen Biotechnology offers flexible customization options for many of its assay kits and detection reagents, including custom lot sizes, bulk ordering, and application-specific formulation adjustments. Volume pricing, custom packaging, and kit bundling may be available depending on the product and intended workflow. A Certificate of Analysis (CoA) and lot-specific QC data are provided with every order. For inquiries regarding large-volume orders, custom configurations, or integration into automated workflows, please contact the BioHippo team for a tailored quotation.
