| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human Histone H2B (acetyl K16) was used as the immunogen for the Histone H2B (acetyl K16) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
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Overview
Histone H2B (acetyl K16) Antibody / HIST2H2BE is a anti-H2B Rabbit antibody Recombinant Rabbit Monoclonal clone 31H96 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: H2B
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31H96, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
Histone H2B (acetyl K16) antibody is widely applied in epigenetics, chromatin biology, and cancer research. By detecting acetylation at this specific site, researchers can examine how histone modifications regulate transcription and genome stability. This modification has been linked to gene activation during cell cycle progression, developmental processes, and stress responses.
Chromatin immunoprecipitation with Histone H2B (acetyl K16) antibody isolates genomic regions enriched in this modification, providing insight into transcriptional regulation. Western blotting detects acetylated H2B protein, while immunofluorescence highlights nuclear localization and distribution of acetylated histones. These methods allow comprehensive analysis of chromatin modifications in diverse contexts.
Histone acetylation at lysine 16 plays a role in DNA repair, particularly homologous recombination, by opening chromatin structure at sites of damage. Dysregulation of acetylation is implicated in oncogenesis, neurodegeneration, and developmental disorders. Histone H2B (acetyl K16) antibody supports studies investigating how epigenetic regulation contributes to disease and therapeutic strategies targeting histone modifiers.
Epigenetic therapies, including histone deacetylase inhibitors, influence acetylation levels at lysine 16 and other residues. By applying this antibody, scientists can evaluate how drugs alter chromatin structure and gene expression. This provides both mechanistic and translational value for cancer and neurological disease research.
Histone H2B (acetyl K16) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
