| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human Histone H2B (crotonyl K12) was used as the immunogen for the Histone H2B (crotonyl K12) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Histone H2B (crotonyl K12) Antibody / HIST2H2BE is a anti-H2B Rabbit antibody Recombinant Rabbit Monoclonal clone 31H73 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: H2B
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31H73, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
Histone H2B (crotonyl K12) antibody is widely applied in epigenetics, chromatin biology, and cancer research. By detecting crotonylation at this specific residue, researchers can examine how histone modifications extend beyond acetylation and methylation to influence transcription. Crotonylation at K12 has been associated with gene activation in embryonic stem cells, immune regulation, and cellular stress adaptation.
ChIP with Histone H2B (crotonyl K12) antibody identifies promoter and enhancer regions enriched in this mark. Western blotting distinguishes crotonylated H2B from unmodified protein, while immunofluorescence highlights nuclear distribution of modified nucleosomes. These applications provide robust methods for exploring the biological relevance of histone crotonylation.
Histone crotonylation is regulated by histone acetyltransferases and deacetylases, many of which also act on acetylation. This overlap suggests coordination between acylation states in regulating gene activity. Dysregulation of crotonylation has been implicated in cancer, neurodegeneration, and metabolic disease. Histone H2B (crotonyl K12) antibody supports studies into how this modification contributes to disease pathology and therapeutic opportunities.
Histone H2B (crotonyl K12) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
