| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Histone H3 (acetyl K36) was used as the immunogen for the Histone H3 (acetyl K36) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Histone H3 (acetyl K36) Antibody / HIST1H3A is a anti-H3 Rabbit antibody Recombinant Rabbit Monoclonal clone 31H90 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: H3
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31H90, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
Histone acetylation neutralizes positive charges on lysine residues, weakening histone-DNA interactions and making chromatin more accessible to transcription factors. Acetylation of lysine 36 in Histone H3 has been shown to facilitate RNA polymerase II elongation and promote recruitment of chromatin remodeling complexes. Studies with Histone H3 (acetyl K36) antibody have revealed that this modification marks actively transcribed genes and is enriched in gene bodies, distinguishing it from promoter-associated acetylations. This underscores its importance in transcriptional fidelity and elongation control.
Dysregulation of Histone H3 acetylation is linked to cancer, developmental disorders, and neurodegeneration. Altered acetylation patterns at lysine 36 contribute to abnormal gene expression in cancers such as leukemia and glioblastoma. Research using Histone H3 (acetyl K36) antibody has shown that histone acetyltransferases and deacetylases targeting this site are often misregulated in tumors, providing potential therapeutic targets. In neurobiology, reduced acetylation has been linked to impaired memory and synaptic plasticity, highlighting its role in cognition and brain function.
Histone H3 (acetyl K36) antibody is applied in chromatin immunoprecipitation, western blotting, and immunofluorescence. Chromatin immunoprecipitation identifies genomic regions marked by acetylation, providing insights into gene regulation. Western blotting confirms acetylation status in whole-cell lysates, while immunofluorescence visualizes global distribution patterns in nuclei. These applications make the antibody essential for epigenetic and transcriptional research.
By supplying validated Histone H3 (acetyl K36) antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
