| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Histone H3 recombinant protein (Position: Q56�R117) was used as the immunogen for the Histone H3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Histone H3 Antibody / HIST1H3A/B/C/D/E/F/G/H/I/J is a anti-H3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: H3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, IHC, ICC, WB
Biological background
Functionally, Histone H3 antibody identifies a 136-amino-acid nuclear protein that forms an octameric core complex with histones H2A, H2B, and H4. This complex organizes 147 base pairs of DNA into nucleosomes, the fundamental repeating unit of chromatin. Modifications of histone H3, including methylation, acetylation, phosphorylation, and ubiquitination, act as epigenetic marks that determine chromatin accessibility and gene expression status. The N-terminal tail of H3 serves as the primary site for these covalent modifications.
The HIST1H3 family of genes is clustered on chromosome 6p22.2, encoding nearly identical variants of histone H3 proteins that differ slightly in sequence and expression timing. Histone H3 variants, including H3.1, H3.2, and H3.3, exhibit distinct deposition patterns; replication-dependent variants incorporate during DNA synthesis, while H3.3 is incorporated independently of replication to maintain transcriptionally active chromatin. These variants play unique roles in genome stability, DNA repair, and transcriptional memory.
Epigenetically, histone H3 modifications define specific chromatin states: trimethylation at lysine 4 (H3K4me3) marks active promoters, while methylation at lysine 9 (H3K9me3) and lysine 27 (H3K27me3) signify repressed or heterochromatic regions. Phosphorylation of serine 10 (H3S10ph) correlates with mitotic chromatin condensation. Dysregulation of these modifications leads to altered gene expression and contributes to developmental disorders, cancer, and epigenetic diseases.
Histone H3 antibody is widely used in epigenetics, chromatin biology, and transcriptional regulation research. It is suitable for western blotting, chromatin immunoprecipitation, immunofluorescence, and immunohistochemistry to detect histone H3 and its modified forms. This antibody supports studies of chromatin structure, gene expression, and nucleosome dynamics. It also serves as a normalization control in nuclear extract analyses due to its consistent expression across cell types.
Structurally, histone H3 adopts a histone fold domain that facilitates DNA wrapping and histone-histone interactions within the nucleosome. Its flexible N-terminal tail extends from the nucleosome surface and interacts with chromatin modifiers and transcriptional machinery.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
