| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human Histone H3 (di methyl K4) was used as the immunogen for the Histone H3 (di methyl K4) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
Histone H3 (di methyl K4) Antibody / HIST1H3A is a anti-H3 Rabbit antibody Recombinant Rabbit Monoclonal clone 31H99 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: H3
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31H99, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
Di methylation of histone H3 at lysine 4 is often enriched at promoter regions of actively transcribed genes. While tri methylation of lysine 4 strongly marks active promoters, di methylation provides a broader distribution pattern, sometimes marking both active and poised regulatory regions. Studies using Histone H3 (di methyl K4) antibody have revealed its involvement in the recruitment of chromatin remodeling complexes and transcription factors, linking it to fine tuning of gene activation. This modification is placed by SET domain containing methyltransferases, including the MLL/SET1 family, and removed by specific demethylases. The dynamic balance of methylation and demethylation at lysine 4 ensures flexibility in gene expression responses.
Dysregulation of histone H3 lysine 4 methylation is implicated in cancer, developmental syndromes, and neurological disorders. Aberrant patterns of di methylation at promoters can alter transcriptional programs, driving oncogenesis or impairing differentiation. For example, mutations in methyltransferases such as MLL2 disrupt normal H3K4 methylation patterns, resulting in abnormal gene expression signatures in leukemia and solid tumors. Research with Histone H3 (di methyl K4) antibody has been critical for characterizing these altered epigenetic landscapes and understanding their impact on disease progression.
Histone H3 (di methyl K4) antibody is widely used in chromatin immunoprecipitation, western blotting, and immunofluorescence. Chromatin immunoprecipitation coupled with sequencing (ChIP seq) allows genome wide mapping of histone modification patterns, revealing regulatory regions associated with transcriptional control. Western blotting confirms global levels of modification, while immunofluorescence highlights distribution within nuclear chromatin. These applications enable researchers to integrate molecular, genomic, and cellular approaches to study histone modification biology.
Beyond oncology, Histone H3 (di methyl K4) antibody has applications in developmental biology and stem cell research. This modification marks regulatory elements required for lineage commitment and differentiation, making it an important indicator of epigenetic states during cell fate transitions. It is also studied in neurobiology, where changes in histone methylation accompany activity dependent gene expression and synaptic plasticity. By providing high quality reagents such as Histone H3 (di methyl K4) antibody,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
