| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human Histone H3 (di methyl K79) was used as the immunogen for the Histone H3 (di-methyl K79) antibody. |
| Isotype | |
| Product Type | |
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| Reactivity | |
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Overview
Histone H3 (di-methyl K79) Antibody / HIST1H3A is a anti-H3 Rabbit antibody Recombinant Rabbit Monoclonal clone 31H84 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: H3
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31H84, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
Histone H3 (di-methyl K79) antibody is widely used in epigenetics, cancer research, and developmental biology. Detection of H3K79me2 serves as a marker of euchromatin and transcriptionally active regions. By applying this antibody, researchers can study how histone methylation influences chromatin remodeling and gene regulation.
In ChIP assays, Histone H3 (di-methyl K79) antibody enriches for genomic regions with active transcription. Western blot detects histone H3 with specific dimethylation at K79, while immunofluorescence maps nuclear distribution of this epigenetic modification. These applications allow detailed study of gene expression and epigenetic control.
Aberrant H3K79 methylation is implicated in leukemogenesis and other cancers. Fusion proteins such as MLL AF9 recruit DOT1L, leading to abnormal dimethylation and deregulated gene expression. By detecting H3K79me2 with specific antibodies, scientists can investigate mechanisms of oncogenic transformation and therapeutic responses to DOT1L inhibitors.
Beyond cancer, H3K79 methylation regulates developmental programs, DNA damage repair, and cell cycle transitions. Its role in lineage specification makes it a critical marker in stem cell biology. Histone H3 (di-methyl K79) antibody therefore provides a versatile tool for exploring transcriptional regulation in both normal and pathological contexts.
Histone H3 (di-methyl K79) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
