| Field | Specification |
|---|---|
| Target | |
| Alternative names | H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic Peptide |
| Conjugate | |
| Concentration | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Product Overview
This monoclonal antibody, raised in Mouse, recognizes Histone H3 (di methyl K9). It is validated for Western Blot, Immunoprecipitation, Immunohistochemistry.
Validated Applications
| Applications | Western Blot, Immunoprecipitation, Immunohistochemistry |
|---|---|
| WB 1 | 1,000-3,000 IHC 1:200-500 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Synthetic Peptide
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Immunogen | Synthetic Peptide |
| Concentration | 1 mg/mL |
| Observed Band | 15kDa |
| Subcellular Location | Nucleus,Chromosome |
| Swiss-Prot | P68431 |
| Gene ID | 8290 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
Reactivity Notes
Validated reactivity: Human, Rat, Mouse. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for Histone H3 (di methyl K9) research from ELK Biotechnology, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
