| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human Histone H3 (mono methyl K79) was used as the immunogen for the Histone H3 (mono methyl K79) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
Histone H3 (mono methyl K79) Antibody / HIST1H3A is a anti-H3 Rabbit antibody Recombinant Rabbit Monoclonal clone 31H92 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: H3
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31H92, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
Lysine 79 monomethylation is catalyzed by the histone methyltransferase DOT1L. Research using Histone H3 (mono methyl K79) antibody has demonstrated that this modification is associated with active transcription, particularly in coding regions of genes. Unlike acetylation or other methylation marks on histone tails, H3K79 methylation occurs within the globular domain of histone H3, influencing nucleosome structure and stability. This makes it a distinct epigenetic signal in chromatin regulation.
Studies with Histone H3 (mono methyl K79) antibody have revealed roles in development, cell cycle progression, and DNA damage response. Monomethylation at this site contributes to recruitment of DNA repair proteins, maintaining genome stability after genotoxic stress. In development, proper levels of H3K79 methylation are essential for lineage commitment and stem cell differentiation. Dysregulation can disrupt normal transcription programs, leading to disease.
Aberrant H3K79 methylation has been strongly linked to leukemia. Research using Histone H3 (mono methyl K79) antibody has shown that mixed lineage leukemia (MLL) gene rearrangements alter DOT1L activity, resulting in abnormal H3K79 methylation patterns. These changes promote oncogenic transcription programs and block differentiation, driving leukemia progression. Targeting DOT1L is now an active therapeutic strategy, highlighting the clinical relevance of this modification.
Histone H3 (mono methyl K79) antibody is used in chromatin immunoprecipitation, western blotting, and immunofluorescence. Chromatin immunoprecipitation maps genome-wide distribution of this modification, western blotting detects modification-specific forms of histone H3, and immunofluorescence reveals nuclear patterns of methylated chromatin. These applications make the antibody indispensable for epigenetics research.
By supplying validated Histone H3 (mono methyl K79) antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
