| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Histone H4 recombinant protein (Position: A16-R96) was used as the immunogen for the Histone H4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Histone H4 Antibody / H4C1/2/3/4/5/6/8/9/11/12/13/14/15/16 is a anti-H4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: H4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
Histone H4 plays a central role in chromatin stability and accessibility. Its N-terminal tail contains lysine residues that undergo multiple modifications including acetylation, methylation, and phosphorylation, which influence transcriptional activation or repression. The Histone H4 antibody allows detection of both native and modified forms, supporting studies into epigenetic regulation and DNA-templated processes. These modifications are dynamic and essential for replication, repair, and recombination.
During DNA replication, Histone H4 is synthesized and assembled onto nascent DNA to restore chromatin structure. The Histone H4 antibody enables visualization of chromatin assembly and nucleosome spacing in proliferating cells. Dysregulation of H4 expression or modification patterns is associated with aberrant chromatin compaction and altered gene expression, contributing to cancer and developmental disorders. Histone H4 lysine 16 acetylation, for instance, plays a crucial role in chromatin decondensation and transcriptional activation.
Histone H4 interacts with chromatin remodelers, histone chaperones, and modifying enzymes that control nucleosome positioning and dynamics. The Histone H4 antibody is widely used to study histone-protein interactions and to map modification patterns across the genome through chromatin immunoprecipitation (ChIP) assays. Changes in H4 modification landscapes serve as biomarkers for epigenetic reprogramming in response to stress, differentiation, or oncogenic transformation.
The Histone H4 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, producing strong nuclear staining characteristic of chromatin localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
