| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Histone H4 (mono methyl K20) was used as the immunogen for the Histone H4 (mono methyl K20) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Histone H4 (mono methyl K20) Antibody / HIST1H4A is a anti-H4 Rabbit antibody Recombinant Rabbit Monoclonal clone 31H81 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: H4
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31H81, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
Histone H4 monomethylated at lysine 20 is catalyzed by the histone methyltransferase SETD8. Research using Histone H4 (mono methyl K20) antibody has shown that this mark is enriched at active gene promoters and regulatory regions, where it contributes to transcriptional competence. Unlike trimethylation at the same residue, which is associated with repression and heterochromatin formation, monomethylation correlates with active chromatin states. This makes the modification a critical epigenetic signal in fine tuning transcription.
In DNA damage response, Histone H4 K20 methylation recruits repair proteins to damaged sites, ensuring accurate repair of double strand breaks. Studies with Histone H4 (mono methyl K20) antibody have revealed that defects in this modification compromise genome stability and increase sensitivity to genotoxic stress. This underscores the functional significance of H4 K20 monomethylation in maintaining genome integrity.
Dysregulation of Histone H4 K20 methylation has been linked to cancer and developmental disorders. Research using Histone H4 (mono methyl K20) antibody has demonstrated that aberrant methylation patterns alter chromatin accessibility and transcriptional programs, promoting oncogenesis. Loss of SETD8 activity or abnormal regulation of this pathway has been associated with impaired differentiation and developmental abnormalities.
Histone H4 (mono methyl K20) antibody is used in chromatin immunoprecipitation, western blotting, and immunofluorescence. Chromatin immunoprecipitation maps distribution of this modification across the genome, western blotting distinguishes between modified and unmodified forms of Histone H4, and immunofluorescence visualizes nuclear distribution of H4 K20 monomethylation. These experimental approaches make the antibody valuable for studying transcriptional regulation and DNA repair.
By supplying validated Histone H4 (mono methyl K20) antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
