| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human HJURP recombinant protein (Position: R6-K594) was used as the immunogen for the HJURP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HJURP Antibody / Holliday junction recognition protein is a anti-HJURP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nucleus, Nucleolus, Cytoplasm.
Key elements and design rationale
- Target: HJURP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Structurally, HJURP is a 748-amino-acid nuclear protein of approximately 80 kilodaltons containing an N-terminal Scm3-like domain that binds CENP-A/H4 heterodimers and a C-terminal domain responsible for centromere targeting through interaction with Mis18 complex components. HJURP localizes exclusively to centromeres during late telophase and early G1 phase, coordinating with the Mis18alpha/beta complex to mediate chromatin assembly and maintain centromere identity.
The HJURP antibody is widely used in cell cycle, chromatin assembly, and cancer research to study centromere formation, histone variant deposition, and chromosomal stability. Western blot analysis detects an 80 kilodalton band corresponding to HJURP, while immunofluorescence reveals discrete nuclear foci at centromeric regions. This antibody provides a key tool for analyzing chromosome segregation fidelity and CENP-A deposition dynamics.
Functionally, HJURP acts as the exclusive chaperone for CENP-A nucleosome assembly, ensuring the maintenance of centromeric chromatin identity across cell divisions. Loss or depletion of HJURP leads to chromosome mis-segregation, aneuploidy, and genomic instability, hallmark features of tumorigenesis. Elevated HJURP expression has been reported in various cancers and correlates with poor prognosis due to its role in promoting proliferation and mitotic progression. The HJURP antibody supports studies on chromatin inheritance, mitotic regulation, and epigenetic maintenance of centromeres.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
