| Field | Specification |
|---|---|
| Target | |
| Alternative names | 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase)|HMGCR |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | 3-hydroxy-3-methylglutaryl-Coenzyme A reductase |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes HMGCR. It is validated for ELISA, Western Blot, Immunohistochemistry, Immunofluorescence.
Validated Applications
| Applications | ELISA, Western Blot, Immunohistochemistry, Immunofluorescence |
|---|---|
| WB | 1:500 - 1:2000 |
| IHC | 1:50 - 1:200 |
| IF | 1:50 - 1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | 3-hydroxy-3-methylglutaryl-Coenzyme A reductase |
| Purification | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 100 kDa |
| UniProt # | P04035 |
| Gene ID | 3156 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
| Alternative Names | 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase)|HMGCR |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for HMGCR research from Fine Test, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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