| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | FLJ14594; LERN 1; LERN1; Leucine rich repeat and Ig domain containing 1; Leucine rich repeat neuronal 6A; Leucine rich repeat neuronal protein 1; Leucine rich repeat neuronal protein 6A; Leucine-rich repeat and immunoglobilin domain-containing protein 1; Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1; Leucine-rich repeat neuronal protein 1; Leucine-rich repeat neuronal protein 6A; LIGO1_Horse; Lingo 1; LINGO1; LRR and Ig domain containing Nogo Receptor inteHorseing protein; Lrrn 6a; Lrrn6a; Lrrn6a protein; MGC17422; Nogo Receptor interacting protein; PRO227; UNQ201; |
| Assay Time | |
| Assay Type | |
| Detection Range | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Tissue homogenates, cell lysates and other biological fluids. |
| Sensitivity | |
| Target |
Scientific background
BNIP3L (Leucine rich repeat and Ig domain containing 1) is a biologically relevant protein marker measured to support mechanistic studies and biomarker discovery (context dependent).
Protein concentrations can change due to secretion, degradation, cell composition shifts, or post-transcriptional regulation, so ELISA readouts often add information beyond gene expression alone.
Quantitative measurements help compare groups and time points using standardized curves and can be interpreted alongside phenotype and pathway-specific readouts.
Why it matters
- Quantify BNIP3L (Leucine rich repeat and Ig domain containing 1) to compare biological changes across conditions, doses, or time points.
- Generate concentration data from a standard curve to support biomarker and mechanistic studies.
How the ELISA works
Designed for Horse samples, this kit uses a The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse Lingo1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse Lingo1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse Lingo1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse Lingo1 in the samples is then determined by comparing the OD of the samples to the standard curve.. After binding and washing, signal is converted to concentration using a standard curve.
Sample types: Tissue homogenates, cell lysates and other biological fluids.
- Detection range: 0.16-10 ng/mL
- Sensitivity/LoD: 0.097 ng/mL
- Assay time: 3.5h
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