| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A human recombinant partial protein (amino acids E12-Q357) was used as the immunogen for the HPD antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HPD Antibody / 4HPPD / 4-hydroxyphenylpyruvate dioxygenase is a anti-HPD Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P, Flow cytometry (FACS), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, nuclear speckles.
Key elements and design rationale
- Target: HPD
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P, FACS, IF, ELISA
Biological background
HPD is highly expressed in the liver and kidneys, organs that are central to amino acid catabolism. Its activity ensures that tyrosine is efficiently broken down, preventing the accumulation of toxic intermediates. Mutations in the HPD gene are associated with tyrosinemia type III, a rare metabolic disorder characterized by elevated levels of tyrosine in the blood and neurological symptoms. Researchers employ a HPD antibody to investigate enzyme function, tissue distribution, and the molecular basis of metabolic diseases.
Beyond its role in metabolism, HPD has clinical importance as a therapeutic target. Small-molecule inhibitors of HPD are being explored to reduce toxic metabolite accumulation in tyrosinemia type I, where blocking this enzyme helps redirect metabolic flux. Using a HPD antibody supports translational research into such therapies by enabling detailed study of enzyme expression and regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
