| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human HPGD recombinant protein (Position: A13-Q266) was used as the immunogen for the HPGD antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HPGD Antibody / 15-hydroxyprostaglandin dehydrogenase is a anti-HPGD Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Nucleus.
Key elements and design rationale
- Target: HPGD
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
Structurally, HPGD is a cytosolic enzyme of approximately 29 kDa that functions as a homodimer. It belongs to the short-chain dehydrogenase/reductase (SDR) family and requires NAD+ as a cofactor for catalytic activity. Its active site includes conserved serine, tyrosine, and lysine residues essential for proton transfer and substrate oxidation. Co-localization studies show HPGD present in cytoplasmic and perinuclear regions of epithelial cells and macrophages, consistent with its role in eicosanoid metabolism.
Functionally, HPGD controls the turnover of bioactive prostaglandins such as PGE2, PGF2alpha, and PGD2. By inactivating these lipid mediators, it dampens inflammatory signaling, cell migration, and angiogenesis. HPGD also participates in the prostaglandin catabolic pathway linked to tumor suppression, as loss of HPGD function often leads to prostaglandin accumulation and oncogenic signaling. The enzyme interacts with various prostaglandin transporters and metabolic enzymes, including SLCO2A1 and AKR1C family members, to coordinate prostaglandin clearance.
Deficiency or silencing of HPGD is associated with several diseases, including colon and lung cancer, endometrial carcinoma, and familial digital clubbing caused by elevated prostaglandin E2. Overexpression, conversely, suppresses tumor growth by limiting COX-2-driven signaling. Pathway involvement includes prostaglandin degradation, arachidonic acid metabolism, and inflammation resolution. During development, HPGD contributes to vascular remodeling and placental differentiation.
The HPGD antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
