HRV 3C Protease, His-GST-tags Recombinant

What it means: E. coli — this protein was produced in E. coli, determining its post-translational modification profile and application suitability.

Why it matters:

• Insect cell (Sf9) systems support post-translational processing required for kinase activity and complex formation; HEK293 supports human-like glycosylation; E. coli yields high-quantity non-glycosylated protein.
• For assays measuring native enzymatic activity (kinase, phosphodiesterase, deacetylase), the expression system directly affects the quality and accuracy of results.

How to interpret here: Verify E. coli is compatible with your assay requirements, particularly for enzyme kinetics and cell-based applications.

What it means: N-terminal His-GST-tags — this affinity tag enables one-step chromatographic purification and can serve as a detection epitope.

Why it matters:

• GST-tag enables glutathione-affinity pull-down assays; His-tag enables IMAC and is compatible with anti-His detection; Avi-tag enables site-specific biotinylation for SPR/BLI capture or streptavidin-based assays; Fc-tag enables protein A/G purification and can extend half-life.
• Tag position (N- vs C-terminal) may affect domain accessibility — verify it does not occlude the active site or binding interface.

How to interpret here: Use anti-N antibodies for tag-based detection or capture in pull-down and ELISA assays.

What it means: Not applicable (BSL-1) — this protein has been classified under BSL-1 safety requirements, the lowest biosafety classification.

Why it matters:

• BSL-1 proteins pose no known risk to healthy adult laboratory workers and do not require specialised containment or personal protective equipment beyond standard laboratory practice.
• Confirms suitability for use in standard biochemistry or molecular biology laboratories without institutional BSL-2 or higher facility requirements.

How to interpret here: Standard laboratory safety practices apply. Review the product SDS/Datasheet for handling precautions specific to this compound.

What it means: This protein spans amino acids 1538-1719 of the target protein, covering the region used for expression and purification.

Why it matters:

• The expressed amino acid region determines which functional domains are present — catalytic domains, binding interfaces, or regulatory regions — affecting activity and assay design.
• For fragment proteins, confirm the region includes your domain of interest (active site, binding pocket, substrate site) before ordering.

How to interpret here: Amino acids 1538-1719 are included in this construct. Cross-reference with the UniProt domain annotation to confirm coverage of your target region.

What it means: 1 Unit of HRV 3C Protease cleaves >95% of 100 µg of target protein at 4°C for 16 hours. — specific activity quantifies the catalytic potency of the protein per unit mass.

Why it matters:

• High specific activity indicates correctly folded, fully active protein, reducing the amount required per assay and improving signal-to-noise in screening campaigns.
• Lot-to-lot specific activity consistency ensures reproducible IC50 values across screening runs and between project teams.

How to interpret here: The stated specific activity was determined under defined assay conditions (see datasheet). Optimise substrate concentrations and incubation times to achieve the dynamic range needed for your inhibitor screening application.