Hsc70/HSPA8 polyclonal antibody

SKU:BHA11306153
Overview
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Anti-Hsc70/HSPA8 antibody (Rabbit). Validated for Western Blot, Immunohistochemistry, Immunofluorescence. Reactive against Human, Mouse, Rat, Monkey targets.
Host Species Rabbit
Species Reactivity Human, Mouse, Rat, Monkey
Conjugate Unconjugated
Validated Applications Western Blot, Immunohistochemistry, Immunofluorescence
Purification Method Affinity Purification
Options selector
Catalog no. Size
BS7135-50UL 50 uL
BS7135-100UL 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL, 100 uL
  • Lead time: In Stock at Manufacturer options listed in “Availability Content”; other statuses may take longer.
  • Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at −20°C as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No BS7135
Alternative Names HSPA8;HEL-33;HEL-S-72p;HSC54;HSC70;HSC71;HSP71;HSP73;HSPA10;LAP-1;LAP1;NIP71;Hsc70
Conjugate
  • Unconjugated
Formulation 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Host Rabbit
Immunogen Recombinant fusion protein of human Hsc70/HSPA8(NP_006588.1).
Molecular Weight ~ 72 kDa
Product Type
  • Primary Antibody
Purity > 95% (by SDS-PAGE)
Reactivity
  • Human
  • Mouse
  • Rat
  • Monkey
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Product Overview

Research Use Only

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding.

Validated Applications

Western Blot (WB) 500 - 1:2000|IHC
Immunohistochemistry (IHC) 50 - 1:200|IF/ICC
Immunofluorescence (IF/ICC) 50 - 1:200

Key Specifications

Host Species Rabbit
Species Reactivity Human, Mouse, Rat, Monkey
Conjugate Unconjugated
Purification Affinity Purification
Formulation 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Molecular Weight ~ 72 kDa
Catalog No. BS7135
UniProt P11142
Alternative Names HSPA8;HEL-33;HEL-S-72p;HSC54;HSC70;HSC71;HSP71;HSP73;HSPA10;LAP-1;LAP1;NIP71;Hsc70

Immunogen / Antigen

Recombinant fusion protein of human Hsc70/HSPA8(NP_006588.1).

Reactivity Notes

Polyclonal Antibodies

Storage & Handling

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Safety

For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.

What applications has the Hsc70/HSPA8 polyclonal antibody been validated for?

This antibody has been validated for: Western Blot, Immunohistochemistry, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.

What is the recommended dilution range?

WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Which species does this antibody recognize?

This antibody has been confirmed reactive against: Human, Mouse, Rat, Monkey. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.

What is the immunogen / antigen used to generate this antibody?

Recombinant fusion protein of human Hsc70/HSPA8(NP_006588.1).

How should this antibody be stored and handled?

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.

Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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