Product Overview
Research Use OnlyThe HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells.
Validated Applications
| Western Blot (WB) | 1:1000 |
|---|---|
| Immunoprecipitation (IP) | 1:50~1:200 |
Key Specifications
| Host Species | Mouse |
|---|---|
| Species Reactivity | Human |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | Predicted band size:70KDa Observed band size:70KDa |
| Catalog No. | MB0016 |
| UniProt | P08107 |
| Alternative Names | Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2; HSPA1A; HSPA1; HSX70; HSPA1B; HSP-70; HSP 70 |
Immunogen / Antigen
Synthetic peptide, corresponding to the C-terminus of Human HSP70.
Reactivity Notes
This antibody detects endogenous levels of HSPA1A and does not cross-react with related proteins
Storage & Handling
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot, Immunoprecipitation. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
WB: 1:1000 IP: 1:50~1:200
This antibody has been confirmed reactive against: Human. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Synthetic peptide, corresponding to the C-terminus of Human HSP70.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
