| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | HSP90AA1, HSP90AB1, HSP90-alpha, HSP90-beta, HSP90A, HSP90B, HSP89A, HSP86, HSP84, HSP90Alpha, HSPCA, HSPC1, HSPC2, HSPCB, HSPCAL3, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90-beta, Heat shock 90 kDa protein 1 alpha isoform, Heat shock 84 kDa protein, D6S182, FLJ26984 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant human HSP90alpha; Specificity mapped to amino acids 291-304 |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
HSP90α and HSP90β are the two major cytosolic isoforms of the highly conserved heat shock protein 90 (HSP90) family, essential for maintaining protein homeostasis in all eukaryotic cells. While they share 85% amino acid sequence identity, the isoforms differ in structure and function: HSP90α primarily forms homodimers and is stress-inducible, whereas HSP90β exists mainly as a monomer and is constitutively expressed.
Both isoforms are abundantly expressed in the brain and play critical roles in the folding, maturation, and stabilization of a wide range of client proteins involved in neuronal signaling, synaptic plasticity, and cellular stress responses. These include kinases (e.g., c-Raf), transcription factors (e.g., p53), and hormone receptors—many of which are implicated in neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and Huntington’s disease.
HSP90α and HSP90β function as part of dynamic chaperone complexes, interacting with co-chaperones like Cdc37 and p23. ATP binding drives conformational changes that enable these complexes to stabilize client proteins and prevent their degradation. However, in disease contexts, this protective mechanism can inadvertently preserve toxic protein species, contributing to pathogenesis.
By recognizing both HSP90α and HSP90β, this antibody provides a powerful tool for researchers exploring the dual roles of HSP90 in neuronal health and disease.
1 µg/ml was sufficient for detection of HSP90αβ in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Cite this product varies by variant:
- SMC-135D — Size: 100 ug: HSP90 alpha/beta Antibody (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-135D, RRID: AB_2121063)
- SMC-135D-A390 — Size: 100 ug: HSP90 alpha/beta Antibody: ATTO 390 (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-135D-A390, RRID: AB_2698211)
- SMC-135D-A488 — Size: 100 ug: HSP90 alpha/beta Antibody: ATTO 488 (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-135D-A488, RRID: AB_2698212)
- SMC-135D-A594 — Size: 100 ug: HSP90 alpha/beta Antibody: ATTO 594 (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-135D-A594, RRID: AB_2698214)
- SMC-135D-BI — Size: 100 ug: HSP90 alpha/beta Antibody: Biotin (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-135D-BI, RRID: AB_2698221)
- SMC-135D-FITC — Size: 100 ug: HSP90 alpha/beta Antibody: FITC (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-135D-FITC, RRID: AB_2698222)
- SMC-135S — Size: 12 ug: HSP90 alpha/beta Antibody (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-135S, RRID: AB_2121063)
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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