| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | htpG; b0473; JW0462; Chaperone protein HtpG; Heat shock protein C62.5; Heat shock protein HtpG; High temperature protein G |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Escherichia coli Chaperone protein HtpG protein (1-624AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This polyclonal anti-htpG antibody raised in Rabbit, conjugated with Biotin, tested in ELISA. Designed to detect htpG in Escherichia coli for others research.
Key elements and design rationale
- Immunogen: Recombinant Escherichia coli Chaperone protein HtpG protein (1-624AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- Biotin: Streptavidin/avidin amplification; block endogenous biotin in biotin-rich samples.
Biological background
htpG (also: htpG, b0473, JW0462, Chaperone protein HtpG, Heat shock protein C62.5, Heat shock protein HtpG, High temperature protein G) — studied in Escherichia coli (strain K12) systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current htpG annotation relevant to others.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Escherichia coli; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
htpG is a protein studied in Escherichia coli biology. This polyclonal antibody raised in Rabbit detects htpG in ELISA applications, IgG isotype.
Reported reactive against Escherichia coli. Immunogen from Escherichia coli (strain K12). Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
Biotin: streptavidin/avidin amplification. Block endogenous biotin in biotin-rich samples.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing htpG; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
