| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human HTR3E recombinant protein (Position: V26-R229) was used as the immunogen for the HTR3E antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HTR3E Antibody / 5-hydroxytryptamine receptor 3E is a anti-HTR3E Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: HTR3E
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
5-hydroxytryptamine receptor 3E influences the biophysical and pharmacological characteristics of heteromeric 5-HT3 receptors. Its inclusion in the receptor complex alters ion conductance, ligand sensitivity, and desensitization kinetics. The HTR3E antibody supports detection of this subunit in intestinal epithelium, brainstem, and enteric neurons, where serotonergic signaling regulates motility, secretion, and sensory transmission. Expression in the gastrointestinal tract suggests a potential role in visceral sensory modulation and nausea control.
Polymorphisms in the HTR3E gene have been linked to irritable bowel syndrome, functional dyspepsia, and other serotonin-related gastrointestinal disorders. The HTR3E antibody facilitates analysis of protein expression in these contexts, helping to clarify the molecular basis of serotonin receptor diversity and disease susceptibility. In the nervous system, 5-hydroxytryptamine receptor 3E expression may fine-tune synaptic activity and contribute to central processing of nausea, anxiety, and emesis. Detection using the HTR3E antibody supports studies on how receptor composition affects neurotransmission and pharmacologic responses to 5-HT3 antagonists used in antiemetic therapy.
Beyond physiology, aberrant expression of 5-hydroxytryptamine receptor 3E has been observed in some cancers, suggesting roles in tumor cell signaling and proliferation. The HTR3E antibody allows evaluation of receptor expression in tumor tissues and cultured cells, supporting research into serotonin-mediated growth regulation. Functional studies continue to explore how HTR3E variants influence receptor pharmacodynamics and sensitivity to drugs such as ondansetron and granisetron.
The HTR3E antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry. It provides clear membrane and cytoplasmic staining consistent with receptor localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
