| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human HTRA4 recombinant protein (Position: A31-D464) was used as the immunogen for the HTRA4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HTRA4 Antibody / Serine protease HTRA4 is a anti-HTRA4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: HTRA4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, HTRA4 antibody identifies a 476-amino-acid secreted and intracellular protease that possesses both trypsin-like catalytic activity and PDZ domain-mediated substrate recognition. HTRA4 cleaves misfolded or aggregated proteins to maintain proteostasis and prevent cellular toxicity. It is expressed primarily in the placenta, where it regulates trophoblast differentiation and contributes to placental remodeling. Additionally, HTRA4 can influence mitochondrial homeostasis and apoptosis by degrading components of the mitochondrial protein import machinery during stress conditions.
The HTRA4 gene is located on chromosome 8p13.3 and is highly expressed in placental trophoblasts, heart, and lung. Its expression increases during pregnancy and hypoxia, reflecting roles in stress response and tissue remodeling. Like other HTRA proteases, HTRA4 combines chaperone-like and proteolytic activities to ensure proper protein folding and clearance.
Pathologically, elevated HTRA4 levels have been linked to preeclampsia, where excessive proteolytic activity contributes to endothelial dysfunction and vascular injury. Dysregulation may also play a role in cardiac remodeling, pulmonary hypertension, and neurodegenerative disease by altering extracellular matrix integrity and stress signaling. Research using HTRA4 antibody supports studies in placental biology, protease function, and oxidative stress response.
HTRA4 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect serine proteases involved in protein homeostasis.
Structurally, Serine protease HTRA4 contains an N-terminal signal peptide, a trypsin-like catalytic domain with the conserved serine-histidine-aspartate triad, and a C-terminal PDZ domain that mediates substrate recognition and oligomerization. These structural features enable substrate-specific proteolysis and regulated activation under stress. This antibody facilitates analysis of HTRA4's function in proteostasis, placental health, and cardiovascular pathology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
