| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | AKR1B10; AKR1B11; AKR1B12; ALDRLn; ARL-1; ARL1; HIS; HSI; MGC14103; aldo-keto reductase family 1; member B10; member B11 (aldose reductase-like); aldose reductase-like 1; aldose re |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Shipping | |
| Storage |
Features & Benefits
Human Aldo-keto reductase family 1 member B10 (AKR1B10) ELISA Kit has high sensitivity and excellent specificity for detection of Human AKR1B10. No significant cross-reactivity or interference between Human AKR1B10 and analogues was observed.
Background
Aldose reductase (AR) is an NADPH-dependent enzyme that was first identified by its ability to reduce glucose to sorbitol. The deduced 316-amino acid AKR1B10 protein shares 71% amino acid identity with AR. Northern blot analysis revealed that AKR1B10 is overexpressed in 54% of HCCs, while AR is overexpressed in 29% of HCCs. Northern blot analysis of normal tissues showed that unlike the ubiquitously expressed AR, AKR1B10 is expressed most abundantly in small intestine and colon, with lower levels in liver, thymus, prostate, testis, and skeletal muscle. AR and ARL1 reduce a similar spectrum of aromatic and aliphatic aldehyde substrates.Highest expression of AKR1B10 in adrenal gland, with modest expression in stomach, placenta, small intestine, and pancreas, and lower expression in all other tissues tested.
This Aldo-keto reductase family 1 member B10 (AKR1B10) ELISA kit is validated for use with Cell culture supernatants, Serum, Plasma, Other biological fluids. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a sandwich ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the sandwich format, the target analyte Aldo-keto reductase family 1 member B10 (AKR1B10) captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Human. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
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