| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | ACAP1; CENTB1; KIAA0050; Arf GAP with coiled coil; ANK repeat and PH domains 1; centaurin beta1; centaurin; beta 1 |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Shipping | |
| Storage |
Features & Benefits
Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACAP1. No significant cross-reactivity or interference between Human ACAP1 and analogues was observed.
Background
ACAP1 and ACAP2 were recruited to platelet-derived growth factor (PDGF)-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. The deduced protein contains 740 amino acids and shares 52% sequence identity with CENTB2. highest expression in spleen, thymus, and peripheral blood leukocytes, intermediate expression in lung, testis, and small intestine, and weak expression in prostate, ovary, and colon. No expression was detected in heart, brain, placenta, liver, skeletal muscle, kidney, and pancreas, and no expression was detected in HeLa cells.ACAP1 shares significant similarity with CENTB2, which the authors called ACAP2, and 95% identity with mouse Acap1.
This Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) ELISA kit is validated for use with Cell culture supernatants, Serum, Plasma, Other biological fluids. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a sandwich ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the sandwich format, the target analyte Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Human. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
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