| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | ANF; ANP; ATFB6; CDD-ANF; PND; atrial natriuretic factor|atriopeptin|cardionatrin|prepronatriodilatin; natriuretic peptides A(NPPA) |
| Product Type | |
| Sensitivity | |
| UniProt # |
Assay Principle
Sandwich ELISA
This kit uses a two-site sandwich ELISA to quantitate ANP (NPPA) in human samples. The plate is pre-coated with anti-ANP capture antibody. Samples and standards bind the capture antibody; biotinylated detection antibody and Streptavidin-HRP are applied; TMB substrate generates colorimetric signal at 450 nm proportional to ANP concentration. Sensitivity: 7.6 pg/mL; range: 15.6–1,000 pg/mL.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Species Reactivity | Human (Homo sapiens) |
| Target Analyte | ANP (Atrial Natriuretic Peptide) / NPPA gene product |
| UniProt ID | P01160 |
| Sensitivity (LLOD) | 7.6 pg/mL |
| Detection Range | 15.6–1,000 pg/mL |
| Sample Types | ⚠ Not specified — confirm with manufacturer before use |
| Plate Format | 96-well, available as 48T or 96T |
| Kit Stability | ≤5% activity loss within stated shelf life |
| Manual | Available (PDF, see Documents tab) |
Standard Kit Components
| Component | Description |
|---|---|
| Microplate | 96-well pre-coated with capture antibody (12×8-well strips) |
| Standards | Recombinant ANP protein for standard curve |
| Detection Antibody | Biotinylated anti-ANP detection antibody |
| Streptavidin-HRP | Enzyme conjugate for signal amplification |
| TMB Substrate | 3,3′,5,5′-Tetramethylbenzidine chromogenic substrate |
| Stop Solution | Sulfuric acid stop solution |
| Wash Buffer | Concentrated wash buffer (dilute before use) |
| Sample Diluent | For sample preparation and dilution |
Scientific Background
Atrial Natriuretic Peptide (ANP), encoded by the NPPA gene (UniProt P01160), is a 28-amino acid cardiac hormone secreted by atrial cardiomyocytes in response to increased atrial wall stress (volume overload, elevated filling pressure). ANP exerts natriuretic, diuretic, and vasodilatory effects, opposing the renin-angiotensin-aldosterone system (RAAS) to reduce blood pressure and cardiac preload. Plasma ANP is markedly elevated in heart failure, atrial fibrillation, and hypertension, making it a validated cardiac biomarker alongside BNP and NT-proBNP. Also known as: ANF, ATFB6, CDD-ANF, PND; atrial natriuretic factor; atriopeptin; cardionatrin.
Safety & Handling
Research Use Only (RUO). Not for diagnostic or therapeutic applications. TMB substrate and stop solution are corrosive — wear appropriate PPE. Sodium azide may be present — avoid ingestion.
Related Products
Browse additional ELISA kits and immunoassay reagents in the BioHippo catalog.
ANP (Atrial Natriuretic Peptide), encoded by NPPA (UniProt P01160), is a 28-amino acid hormone released from atrial cardiomyocytes in response to atrial stretch (volume overload, elevated filling pressure). It promotes natriuresis, diuresis, and vasodilation to reduce cardiac preload. Plasma ANP is elevated in heart failure, atrial fibrillation, and hypertension, making it a clinically relevant research biomarker.
Sensitivity is 7.6 pg/mL. Normal human plasma ANP is 20–77 pg/mL in healthy adults — comfortably within the detectable range (15.6–1,000 pg/mL) and well above the LLOD. Samples from heart failure or fluid overload subjects may exceed 100 pg/mL — prepare 2–5-fold dilutions if OD exceeds the upper standard.
Not explicitly listed — consult the product manual (Documents tab) for validated matrices. Standard ANP ELISA formats are typically validated for EDTA-plasma, serum, and urine. ANP is susceptible to proteolysis — collect blood in EDTA tubes on ice and process immediately; add protease inhibitors for tissue samples.
Prepare an 8-point curve by 2-fold serial dilution of the provided ANP standard from 1,000 pg/mL to 15.6 pg/mL, plus a zero blank. Run in duplicate. Read at 450 nm (reference 570 nm or 540 nm). Fit with a 4-parameter logistic (4PL) model for accurate interpolation.
Yes. ANP and BNP/NT-proBNP assays are independent and can be run on the same sample set in parallel. ANP reflects atrial stretch and preload; BNP/NT-proBNP primarily reflect ventricular wall stress. Running both provides a more complete picture of cardiac filling pressures and natriuretic peptide biology.
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