| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bone morphogenetic protein 2 ;BMP2 ; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: CHO; Immunogen sequence: Q283-R396 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Tissue, Cell Culture Supernatant, Serum |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human BMP2 in bone tissue, cell culture supernatants and serum. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Kit Components
| Anti-Human BMP2 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human BMP2 Standard | 2 vials, 10 ng/tube |
| Human BMP2 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Bone tissue, cell culture supernatants and serum |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <2 pg/mL |
| Standard | Recombinant human BMP2 (Gln283–Arg396), expressed in CHO |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.3–7.8% (3 samples, n = 16 each) |
| Inter-assay CV | 6.1–7.9% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum samples measured about 50 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.052; 31.2 = 0.104; 62.5 = 0.151; 125 = 0.238; 250 = 0.399; 500 = 0.742; 1000 = 1.246; 2000 = 1.927. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.052 | 0.104 | 0.151 | 0.238 | 0.399 | 0.742 | 1.246 | 1.927 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 55 | 250 | 1005 | 59 | 255 | 1061 |
| Standard deviation | 4.29 | 13.25 | 70.35 | 4.66 | 15.55 | 83.81 |
| CV (%) | 7.8% | 5.3% | 7% | 7.9% | 6.1% | 7.9% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Weizhen Chen et al. (2021) An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties. Biomaterials. 10.1016/j.biomaterials.2021.121260
Fenghe Yang et al. (2022) Biodegradable magnesium incorporated microspheres enable immunomodulation and spatiotemporal drug release for the treatment of osteonecrosis of the femoral head. Composites Part B-Engineering. 10.1016/j.compositesb.2022.110430
Qiang Ao et al. (2020) Fibrin Glue/Fibronectin/Heparin-Based Delivery System of BMP2 Induces Osteogenesis in MC3T3-E1 Cells and Bone Formation in Rat Calvarial Critical-Sized Defects. ACS Applied Materials & Interfaces. 10.1021/acsami.0c01371
Long Li et al. (2014) Controlled dual delivery of BMP-2 and dexamethasone by nanoparticle-embedded electrospun nanofibers for the efficient repair of critical-sized rat calvarial defect. Biomaterials. 10.1016/j.biomaterials.2014.10.015
Tianyi Zhou et al. (2024) An injectable carboxymethyl chitosan-based hydrogel with controlled release of BMP-2 for efficient treatment of bone defects. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2024.137120
Pan Weimin et al. (2013) Tendon-to-bone healing using an injectable calcium phosphate cement combined with bone xenograft/BMP composite. Biomaterials. 10.1016/j.biomaterials.2013.09.018
Chunyan Qiao et al. (2022) Using poly(lactic-co-glycolic acid) microspheres to encapsulate plasmid of bone morphogenetic protein 2/polyethylenimine nanoparticles to promote bone formation in vitro and in vivo. International Journal of Nanomedicine. 10.2147/IJN.S45184
Xiaowei Xu et al. (2019) Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy. Nanoscale. 10.1039/C9NR07017F
Hongjuan Cao et al. (2026) Injectable, regenerative and anti-infective PEGylated polyglycerol sebacate-modified calcium phosphate cements triggered by berberine and rhBMP-2 for oral bone defect repair. Dental Materials. 10.1016/j.dental.2026.01.012
Zhenzhen Wu et al. (2020) The synergetic effect of bioactive molecule–loaded electrospun core-shell fibres for reconstruction of critical-sized calvarial bone defect—The effect of synergetic release on bone Formation. Cell Proliferation. 10.1111/cpr.12796
