| Field | Specification |
|---|---|
| Mfr No | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Optimized DNA sequence encoding extracellular domain of human CD48including a C-terminal 6His tag was expressed in HEK293 cells. |
| Product Type | |
| Purity | |
| Storage | |
| UniProt # |
Product Overview
Research-grade Peptide Reagent: Human CD48 protein (Recombinant) (STJP000399).
Target & Specificity
| Gene Symbol | CD48 |
|---|---|
| NCBI Gene ID | 962 |
| UniProt ID | P09326 |
| Immunogen | Optimized DNA sequence encoding extracellular domain of human CD48including a C-terminal 6His tag was expressed in HEK293 cells. |
| Immunogen Region (aa) | ECD |
| Antibody Production Host | HEK293 |
| Conjugate | Unconjugated |
This peptide is designed for use specifically with the paired STJ-series antibody against the same target epitope. Cross-reactivity with other targets has not been evaluated and should be determined empirically.
Specifications
| Purity | >95%, as determined by SDS-PAGE and HPLC |
|---|---|
| Formulation | 0.2 Mu m filtered PBS solution, pH7.2. |
| Storage | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. |
| Validated Applications | Western Blot|Immunodepletion |
Recommended Use
For WB: load 5–20 ng per lane alongside experimental samples to confirm antibody function and expected band size. For IHC: apply to a positive-control tissue section at the concentration recommended for the paired antibody.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorSt John's Laboratory LtdCatalog No.STJP000399UniProtP09326
Load 5–20 ng per WB lane alongside experimental samples to confirm anti-CD48 antibody performance. For IHC, apply to a positive-control tissue section alongside experimental sections. Pair with antibody STJP000399 for matched validation.
For WB: 5–20 ng per lane produces a clear band under standard conditions. For IHC: use the concentration specified on the antibody datasheet. If no signal: confirm antibody is within expiry and antigen retrieval conditions are optimal.
This peptide is designed to pair with antibody STJP000399. Immunogen region: Optimized DNA sequence encoding extracellular domain of human CD48including a C-terminal 6His tag was expressed in HEK293 cells.. Using with a different antibody clone may not produce blocking results if epitopes differ.
Validated for Western Blot, Immunodepletion specificity controls. Performance in other applications (ELISA, IP) requires independent validation.
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles.. Purity: >95% by HPLC. Prepare single-use aliquots to avoid freeze-thaw; stable ≥12 months at −20 °C when lyophilized.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
