Human Glutamatergic Neurons (iPSC derived, GFP labeled, Normal)

Overview

Human Glutamatergic Neurons (iPSC derived, GFP labeled, Normal) is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Neural within the Nervous system.

The rapid and highly reproducible generation of mature and functioning neurons from human pluripotent stem cells (hPSCs), neural progenitors, or fibroblasts has been made possible through the utilization of transcription factors. These factors have played a pivotal role in facilitating investigations related to neurodevelopment, disease modeling, drug screening, and neuronal replacement therapies. By employing various combinations of transcription factors along with specific small molecules, researchers have successfully generated populations of glutamatergic neurons [1,2] .This significant breakthrough has provided valuable insights into neural processes and offered potential avenues for therapeutic advancements. This significant breakthrough has provided valuable insights into neural processes and offered potential avenues for therapeutic advancements.iXCells Biotechnologies takes pride in offering fully differentiated and functional human iPSC-derived glutamatergic neurons that exhibit typical neuronal morphology and express key markers associated with glutamatergic identity, such as VGLUT2, MAP2, and TUJ1 (Figure 1A, B). These neurons not only display robust neural activity but also show an increase in activity over time, indicating progressive maturation (Figure 1C, D). When cultured in the Human Glutamatergic Neuron Maturation Media (Cat# MD-0116) , our neurons provide a reliable model for studying neuronal function and responses (Figure 2). Additionally, our iPSC-derived neurons can be co-cultured with glial cells, enabling the development of comprehensive drug screening platforms to evaluate drug efficacy, neurotoxicity, and other neural responses. iXCells Biotechnologies takes pride in offering fully differentiated and functional human iPSC-derived glutamatergic neurons that exhibit typical neuronal morphology and express key markers associated with glutamatergic identity, such as VGLUT2, MAP2, and TUJ1 (Figure 1A, B). These neurons not only display robust neural activity but also show an increase in activity over time, indicating progressive maturation (Figure 1C, D). When cultured in the Human Glutamatergic Neuron Maturation Media (Cat# MD-0116), our neurons provide a reliable model for studying neuronal function and responses (Figure 2). Additionally, our iPSC-derived neurons can be co-cultured with glial cells, enabling the development of comprehensive drug screening platforms to evaluate drug efficacy, neurotoxicity, and other neural responses. Figure 1. Human iPSCs-derived glutamatergic neurons show expression of characteristic biological markers. (A) Immunostaining shows the expression of neuronal makers MAP2 and Tuj1 at 5 days post-thawing. (B) Immunostaining shows expression of glutamatergic marker VGLUT2, 25 days post-thawing Scale bar 30μm. (C) Glutamatergic neurons were differentiated as a monolayer on a MEA plate and co-cultured with iPSC-derived Astrocytes (40HU-008) and subsequently neuronal activity was measured. (D) The electrical parameters were measured in the MEA over time and the results are graphed as mean ± SEM for mean firing rate and number of spikes..

Key elements and design rationale

• Cell identity: Neural (iPSC-Derived Cells)
• Source context: Glutamatergic Neurons; Nervous
• Biosafety level: BSL-2 (follow your institution’s biosafety program and local regulations)
• Growth properties: Adherent

Product-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.

Biological background

Neural and glial cell models support studies of neuronal signaling, synaptic biology, neuroinflammation, and cell-type–specific responses to injury or disease-relevant stimuli.

Across primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.

Research relevance and current trends

• Increasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.
• Adoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.
• Integration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.
• Growth of human-relevant neural models (including glial components) to study circuit- and inflammation-linked phenotypes.

Common research applications

• Profile identity markers by flow cytometry or immunostaining in cultured cells
• Quantify neurite outgrowth and synaptic marker profiles in neural cultures
• Quantify functional responses to defined stimuli relevant to the model system
• Compare baseline phenotype across donors/conditions using gene expression profiling
• Measure neuroinflammatory signaling in neuron–glia or microglia-enriched models

Interpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.

Notes for experimental interpretation

• Donor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.
• Passage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.
• Contamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.
• Use appropriate negative/positive controls for the readout (e.g., unstimulated controls, pathway agonists/antagonists) to contextualize observed changes.

SKU:BHC18500315

Customization & Add-ons: Can't find the cell line you need—or require a custom cell-based solution for your project? We can help you source the best match or support custom cell line services for diverse research needs, including cell line sourcing and selection (species, tissue, and disease model matching), stable cell line engineering (overexpression, knockdown, or knockout via CRISPR/Cas9, shRNA, or sgRNA), reporter gene integration (GFP, RFP, luciferase, and other fluorescent or bioluminescent constructs), genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles), inducible expression systems (Tet-On/Off and other regulatable constructs), drug resistance marker selection (puromycin, G418, hygromycin, and others), custom growth and media optimisation for specific assay requirements, scale-up production for high-throughput screening campaigns, and authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.