| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Sensitivity |
Product Overview
Sandwich ELISA KitLow-density lipoprotein (LDL) is a type of lipoprotein that transports cholesterol and triglycerides from the liver to peripheral tissues. LDL is one of the five major groups of lipoproteins; these groups include chylomicrons, very low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), low-density lipoprotein, and high-density lipoprotein (HDL). Like all lipoproteins, LDL enables fats and cholesterol to move within the water based solution of the blood stream. LDL also regulates…
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate Gly-LDL in samples. An antibody specific for Gly-LDL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGly-LDL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Gly-LDL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbo…
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Method | Sandwich ELISA |
| Detection Range | 7.8-500 ng/mL |
Stability & Storage
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Bio…
Safety & Handling
Related Products
Browse our complete selection of ELISA kits, antibodies, and assay reagents across species at BioHippo.
This is a sandwich ELISA kit. It employs a capture antibody pre-coated on the microplate surface and a biotin-conjugated detection antibody. After incubation with streptavidin-HRP and TMB substrate, a colorimetric signal proportional to analyte concentration is generated and measured at 450 nm.
The linear detection range of this kit is 7.8-500 ng/mL. Samples exceeding the upper limit must be diluted prior to testing. Samples below the lower limit may require concentration steps. Always verify that interpolated values fall within the linear portion of the standard curve.
This kit is validated for use with serum and plasma samples. Cell culture supernatants and tissue homogenates may also be tested after appropriate preparation. Avoid grossly hemolyzed, lipemic, or bacterially contaminated samples, as these may produce inaccurate results.
A microplate reader capable of measuring absorbance at 450 nm (with a 540–570 nm reference wavelength) is required. A plate washer is recommended for consistent washing steps. Calibrated micropipettes, incubator (37°C), and standard laboratory consumables are also needed.
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O… Prepare working reagents fresh for each run. Do not freeze kit components unless specifically directed in the insert.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
