| Field | Specification |
|---|---|
| Target | |
| Gene ID | |
| UniProt # | |
| Species | |
| Reactivity | |
| Applications | |
| Assay type | |
| Sample type(s) | Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Detection method | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
HMGB1 (high mobility group box 1) is a highly conserved nuclear protein that acts as a chromatin-binding factor and, when released from damaged or activated cells, as a potent damage-associated molecular pattern (DAMP) driving inflammation. Circulating HMGB1 is studied as a biomarker in sepsis, sterile injury, autoimmunity, and cancer.
In this sandwich-format assay, a plate pre-coated with a capture antibody specific for HMGB1 (High mobility group protein B1) binds analyte from the sample; a biotinylated detection antibody and HRP-conjugate then form the detection complex, and color development is proportional to analyte concentration.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Assay Time | 3.5 hours |
| Detection Range | 31.25~2000pg/mL |
| Sensitivity | 18.75pg/mL |
| Detection Method | Colorimetric |
| Sample Types | Serum, Plasma, Other Biological Fluids |
| Reactivity | Human |
| Storage | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Specificity | This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed. |
| Intra-assay Precision (CV) | 5.88%, 4.25%, 5.13% (three sample levels) |
| Inter-assay Precision (CV) | 5.38%, 4.69%, 3.49% (three sample levels) |
| Recovery | Serum 94-108%; EDTA plasma 94-107%; cell culture media 93-105% |
| Shelf Life | Individual components: 6 months when stored separately according to the manual |
| UniProt ID | HMGB1_HUMAN |
Kit Components
Kit contents as listed by the manufacturer (component quantities shown for 48T / 96T where applicable):
- Micro ELISA Plate (96-well, dismountable)
- Reference Standard
- Biotinylated Detection Antibody (concentrated 100×)
- HRP Conjugate (concentrated 100×)
- Reference Standard & Sample Diluent
- Biotinylated Detection Ab Diluent
- HRP Conjugate Diluent
- Wash Buffer (concentrated 25×)
- Substrate Reagent
- Stop Solution
- Plate Sealers
- Manual and Certificate of Analysis
Assay Workflow
The kit provides the components required for standard-curve quantification, including pre-coated microplate strips, standards, conjugate, substrate, and wash and stop solutions (see the kit manual for the full component table). Absorbance is read on a standard microplate reader, and analyte concentrations are interpolated from the standard curve. Total hands-on-to-result time is approximately 3.5 hours.
Safety & Handling
For scientific research use only (RUO). This kit must not be used in diagnostic, therapeutic, or other medical applications. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Human ELISA kits and matched research antibodies from St John's Laboratory Ltd, or contact us to source related assays for HMGB1 research.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
