| Field | Specification |
|---|---|
| Target | |
| Alternative names | Insulin-like growth factor I; IGF-I; Mechano growth factor; MGF; Somatomedin-C; IGF1; IBP1 |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: G49-A118 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human insulin-like growth factor 1 (gene IGF1) in cell culture supernatants, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Insulin-like growth factor 1 (IGF-1) is a peptide hormone, produced mainly by the liver under growth hormone control, that is structurally related to insulin. Acting through the IGF-1 receptor, it promotes cell growth, proliferation and survival and mediates many effects of growth hormone on body growth and metabolism.
Kit Components
| Anti-Human IGF1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human IGF1 Standard | 2 vials, 10 ng/tube |
| Human IGF1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <50 pg/mL |
| Standard | Recombinant human IGF1 (Gly49–Ala118), expressed in E. coli |
| Cross-reactivity | No detectable cross-reactivity with IGF-2 |
| Intra-assay CV | 4.1–4.6% (3 samples, n = 16 each) |
| Inter-assay CV | 5.1–6.7% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:4. In the supplier’s tests, serum and plasma samples measured about 2–6 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.046; 156 = 0.087; 312 = 0.128; 625 = 0.215; 1250 = 0.393; 2500 = 0.674; 5000 = 1.232; 10000 = 1.803. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.046 | 0.087 | 0.128 | 0.215 | 0.393 | 0.674 | 1.232 | 1.803 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 215 | 1065 | 3566 | 284 | 1154 | 4861 |
| Standard deviation | 8.82 | 45.80 | 164.04 | 14.48 | 68.09 | 325.69 |
| CV (%) | 4.1% | 4.3% | 4.6% | 5.1% | 5.9% | 6.7% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Activating reagent preparation
IGF-1 is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant IGF-1.
Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H2O.
Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H2O, add H2O to adjust volume to 100ml.
Sample activation procedure
Activate the sample
Cell culture supernate: add activating reagent pro rata, i.e. add 20ul of Solution A into 100ul of sample, 10 min later, add 20ul of Solution B. PH 7.0-7.6.
Serum, plasma (heparin, EDTA): add activating reagent pro rata, i.e. add 20ul of Solution A into 40ul of sample, 10 min later, add 20ul of Solution B. PH 7.0-7.6.
It is unnecessary to activate the recombinant IGF-1.
Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Shimin Tao et al. (2025) Computational Toxicology to Elucidate PFASs Causing Fetal Growth Restriction via Binding to and Degrading IGF1 Protein. Environmental Science & Technology. 10.1021/acs.est.5c05620
Ni Fang et al. (2013) IGF-1 promotes the development and cytotoxic activity of human NK cells. Nature Communications. 10.1038/ncomms2484
Yu Nanlan et al. (2020) Twist1 Contributes to the Maintenance of Some Biological Properties of Dermal Papilla Cells in vitro by Forming a Complex With Tcf4 and β-Catenin. Frontiers in Cell and Developmental Biology. 10.3389/fcell.2020.00824
Lippolis Catia et al. (2015) Resistance to multikinase inhibitor actions mediated by insulin like growth factor-1. Journal of Experimental & Clinical Cancer Research. 10.1186/s13046-015-0210-1
Zhigang Wang et al. (2022) Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer. OncoTargets and Therapy. 10.2147/OTT.S51997
Xin Tang et al. (2023) Adipose-Derived Stem Cell Exosomes Antagonize the Inhibitory Effect of Dihydrotestosterone on Hair Follicle Growth by Activating Wnt/β-Catenin Pathway. Stem Cells International. 10.1155/2023/5548112
Gilson P Dorneles et al. (2020) Immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial. Scientific Reports. 10.1038/s41598-020-78750-z
Hao Huang et al. (2022) Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulating HIF-1α and VEGF. Annals of Translational Medicine. 10.21037/atm-21-6812
Wang Huanyu et al. (2024) Increased serum carboxylesterase-1 levels are associated with metabolic dysfunction associated steatotic liver disease and metabolic syndrome in children with obesity. Italian Journal of Pediatrics. 10.1186/s13052-024-01733-7
Kai Tian et al. (2023) Autologous i-PRF promotes healing of radiation-induced skin injury. Wound Repair and Regeneration. 10.1111/wrr.13083
